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mouse multiple tissue northern blot  (TaKaRa)


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    Structured Review

    TaKaRa mouse multiple tissue northern blot
    Mouse Multiple Tissue Northern Blot, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+multiple+tissue+northern+blot/Mouse+Eye+Total+RNA/pm39808388-14-16-23
    Average 95 stars, based on 51 article reviews
    mouse multiple tissue northern blot - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Northern Blot:

    Article Title: Uncoupling protein 5 (UCP5)
    Article Snippet: .. Two human brain multiple tissue Northern blots (Clontech) and a mouse multiple tissue Northern blot (Clontech) were also similarly probed with human UCP5 and mouse UCP5 cDNA probes, respectively. ..

    Article Title: G2/M checkpoint gene expression in developing germ cells.
    Article Snippet: Cell cycle progression is prevented by signal transduction pathways known as checkpoints which are activated in response to replication interference and DNA damage.. We cloned a G2/M cell cycle phase-related checkpoint gene from a neonatal mouse testis cDNA library which was identified as mouse claspin, a proposed adaptor protein for Chk1.. As part of a study on germ cell differentiation we examined the expression of the checkpoint gene, Chk1, and claspin at 12.5 and 14.5 days post coitum (dpc) and in the post-natal phase.

    Article Title: Pancreatic Expression and Mitochondrial Localization of the Progestin-AdipoQ Receptor PAQR10
    Article Snippet: .. A mouse multiple tissue Northern blot (Clontech) was hybridized with a PAQR10-specific cDNA probe as described in Materials and Methods. (B) RT-PCR. ..

    Article Title: Polynucleotides encoding mammalian cytokine-like factor 7
    Article Snippet: .. Mouse Multiple Tissue Northern Blot (Clontech, Palo Alto, Calif.), mouse northern dot blot (Clontech), a mouse embryo northern blot, and a mouse spinal cord dot blot were probed to determine the tissue distribution of murine Zcyto7. .. Mouse Multiple Tissue Northern Blot (Clontech, Palo Alto, Calif.), mouse northern dot blot (Clontech), a mouse embryo northern blot, and a mouse spinal cord dot blot were probed to determine the tissue distribution of murine Zcyto7.

    Article Title: Regulation of Ghrelin Signaling by a Leptin-induced Gene, Negative Regulatory Element-binding Protein, in the Hypothalamic Neurons
    Article Snippet: After separation on 1.2% agarose gels containing 2.4% formaldehyde, total RNA was transferred to positively charged nylon membranes (Roche Diagnostics). .. For the tissue blot analysis, mouse Multiple Tissue Northern blot was obtained from Clontech Laboratories. .. Then, the membranes were hybridized with the 32 P-labeled NREBP probe in a quick hybridization solution (Stratagene, La Jolla, CA) at 68 °C for 2 h. After washing twice in 2× SSC buffer (1× SSC = 44.6 μmol/l sodium chloride, 5 μmol/l trisodium citrate, pH 7.0) containing 0.1% SDS at 68 °C for 15 min, and once in 0.1× SSC buffer containing 0.1% SDS at 68 °C for 20 min, the membranes were exposed to x-ray films for an appropriate period.

    Article Title: Daxx, a Novel Fas-Binding Protein That Activates JNK and Apoptosis
    Article Snippet: .. For Northern analysis, the C-terminal 0.7 kb fragment of murine Daxx and a human β-actin cDNA (Clontech) were used to probe a mouse multiple tissue Northern blot (Clontech), according to the manufacturer's protocol. ..

    Article Title: Differentially Expressed Nedd4-binding Protein Ndfip1 Protects Neurons Against Methamphetamine-induced Neurotoxicity.
    Article Snippet: .. Mouse Multiple Tissue Northern Blot (Clontech) was also used to clarify the distribution of the differentially expressed genes. .. To make radiorabeled anti-sense probes for differentially expressed 8CB + 8CC mRNA, asymmetric PCR was performed using 0.2 pmol of P8 forward primers, and 20 pmol of T8 reverse primers and [α-32P]dCTP (Amersham) under 20 cycles of 94 °C 1 min, 40 °C 1 min, 68 °C 2 min. To make negative control sense probes, asymmetric PCR using 20 pmol of P8 forward primers and 0.2 pmol of T8 reverse primers was also performed.

    Article Title: Differentially Expressed Nedd4-binding Protein Ndfip1 Protects Neurons Against Methamphetamine-induced Neurotoxicity.
    Article Snippet: TRIzol Reagent and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Invitrogen (ThermoFisher Scientific, San Diego, CA, USA). .. A differential display kit (Delta differential display kit), RT-PCR kit (Advantage RT-for-PCR kit), 3’-terminal deoxynucleotidyl transferase, Mouse Multiple Tissue Northern Blot were from Clontech (CA, USA). .. [α-33P]dATP, [α-32P]dCTP and a T7 Sequenase version 2.0 DNA sequencing kit were purchased from Amersham Life Science (IL, USA).

    Dot Blot:

    Article Title: Polynucleotides encoding mammalian cytokine-like factor 7
    Article Snippet: .. Mouse Multiple Tissue Northern Blot (Clontech, Palo Alto, Calif.), mouse northern dot blot (Clontech), a mouse embryo northern blot, and a mouse spinal cord dot blot were probed to determine the tissue distribution of murine Zcyto7. .. Mouse Multiple Tissue Northern Blot (Clontech, Palo Alto, Calif.), mouse northern dot blot (Clontech), a mouse embryo northern blot, and a mouse spinal cord dot blot were probed to determine the tissue distribution of murine Zcyto7.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Differentially Expressed Nedd4-binding Protein Ndfip1 Protects Neurons Against Methamphetamine-induced Neurotoxicity.
    Article Snippet: TRIzol Reagent and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Invitrogen (ThermoFisher Scientific, San Diego, CA, USA). .. A differential display kit (Delta differential display kit), RT-PCR kit (Advantage RT-for-PCR kit), 3’-terminal deoxynucleotidyl transferase, Mouse Multiple Tissue Northern Blot were from Clontech (CA, USA). .. [α-33P]dATP, [α-32P]dCTP and a T7 Sequenase version 2.0 DNA sequencing kit were purchased from Amersham Life Science (IL, USA).



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    (A, B) <t>Northern</t> <t>blot</t> analysis of <t>multiple</t> <t>mouse</t> <t>tissue</t> RNA shows messages for GSK3αand β are highest in testis. (C, D) Quantitative PCR showing increasing Gsk3α and Gsk3β mRNA levels in postnatal developing testis. The results are represented as fold change after normalizing the Gsk3α and Gsk3β mRNA levels with Gapdh mRNA. These data are representative of three independent experiments in triplicates, and error bars represent SE.
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    (A, B) <t>Northern</t> <t>blot</t> analysis of <t>multiple</t> <t>mouse</t> <t>tissue</t> RNA shows messages for GSK3αand β are highest in testis. (C, D) Quantitative PCR showing increasing Gsk3α and Gsk3β mRNA levels in postnatal developing testis. The results are represented as fold change after normalizing the Gsk3α and Gsk3β mRNA levels with Gapdh mRNA. These data are representative of three independent experiments in triplicates, and error bars represent SE.
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    (A, B) <t>Northern</t> <t>blot</t> analysis of <t>multiple</t> <t>mouse</t> <t>tissue</t> RNA shows messages for GSK3αand β are highest in testis. (C, D) Quantitative PCR showing increasing Gsk3α and Gsk3β mRNA levels in postnatal developing testis. The results are represented as fold change after normalizing the Gsk3α and Gsk3β mRNA levels with Gapdh mRNA. These data are representative of three independent experiments in triplicates, and error bars represent SE.
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    TaKaRa mouse embryo multiple tissue northern blot
    (A, B) <t>Northern</t> <t>blot</t> analysis of <t>multiple</t> <t>mouse</t> <t>tissue</t> RNA shows messages for GSK3αand β are highest in testis. (C, D) Quantitative PCR showing increasing Gsk3α and Gsk3β mRNA levels in postnatal developing testis. The results are represented as fold change after normalizing the Gsk3α and Gsk3β mRNA levels with Gapdh mRNA. These data are representative of three independent experiments in triplicates, and error bars represent SE.
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    TaKaRa mouse multiple tissue northern (mtn) blots
    (A, B) <t>Northern</t> <t>blot</t> analysis of <t>multiple</t> <t>mouse</t> <t>tissue</t> RNA shows messages for GSK3αand β are highest in testis. (C, D) Quantitative PCR showing increasing Gsk3α and Gsk3β mRNA levels in postnatal developing testis. The results are represented as fold change after normalizing the Gsk3α and Gsk3β mRNA levels with Gapdh mRNA. These data are representative of three independent experiments in triplicates, and error bars represent SE.
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    Image Search Results


    (A, B) Northern blot analysis of multiple mouse tissue RNA shows messages for GSK3αand β are highest in testis. (C, D) Quantitative PCR showing increasing Gsk3α and Gsk3β mRNA levels in postnatal developing testis. The results are represented as fold change after normalizing the Gsk3α and Gsk3β mRNA levels with Gapdh mRNA. These data are representative of three independent experiments in triplicates, and error bars represent SE.

    Journal: Biology of Reproduction

    Article Title: Isoform-specific requirement for GSK3α in sperm for male fertility †

    doi: 10.1093/biolre/ioy020

    Figure Lengend Snippet: (A, B) Northern blot analysis of multiple mouse tissue RNA shows messages for GSK3αand β are highest in testis. (C, D) Quantitative PCR showing increasing Gsk3α and Gsk3β mRNA levels in postnatal developing testis. The results are represented as fold change after normalizing the Gsk3α and Gsk3β mRNA levels with Gapdh mRNA. These data are representative of three independent experiments in triplicates, and error bars represent SE.

    Article Snippet: Mouse multiple tissue northern blot was obtained from Zyagen.

    Techniques: Northern Blot, Real-time Polymerase Chain Reaction